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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/07/2019 |
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Data da última atualização: |
04/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. |
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Afiliação: |
Universidade Federal de Mato Grosso do Sul/FUFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia; LENITA RAMIRES DOS SANTOS, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. MenosVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellu... Mostrar Tudo |
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Palavras-Chave: |
DNA vaccine; T4SS; VirB operon; VirB12; VirB9. |
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Thesagro: |
Brucella Abortus. |
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Thesaurus Nal: |
Bovine brucellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02847naa a2200289 a 4500
001 2110376
005 2019-07-04
008 2019 bl uuuu u00u1 u #d
100 1 $aCAITANO-BERTOLACCI, M. A. B.
245 $aDNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice.$h[electronic resource]
260 $c2019
520 $aVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection.
650 $aBovine brucellosis
650 $aBrucella Abortus
653 $aDNA vaccine
653 $aT4SS
653 $aVirB operon
653 $aVirB12
653 $aVirB9
700 1 $aBASTOS, R. R. P.
700 1 $aSANTOS, L. R. dos
700 1 $aSOARES, C. O.
700 1 $aRIEGER, J. S. G.
700 1 $aMANTOVANI, C.
700 1 $aSANTANA, M. de S.
700 1 $aROSINHA, G. M. S.
773 $tGenetics and Molecular Research$gv. 18, n. 2, gmr18295, 2019
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
19/09/2023 |
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Data da última atualização: |
19/09/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
ROMANI, L. A. S.; BAMBINI, M. D.; BARIANI, J. M.; DRUCKER, D. P.; MINITTI, A. F.; GONZALEZ, A. F.; KUROMOTO, V. M.; TELLES, G. A. de S.; ARAÚJO, R. F.; DIAS, C. N.; ASSUNÇÃO, B. S. B. de; SOUZA, S. S. de; LUCHIARI JÚNIOR, A.; MEIRA, C. A. A. |
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Afiliação: |
LUCIANA ALVIM SANTOS ROMANI, CNPTIA; MARTHA DELPHINO BAMBINI, CNPTIA; JOICE MACHADO BARIANI, CNPTIA; DEBORA PIGNATARI DRUCKER, CNPTIA; ANDRE FACHINI MINITTI, CNPTIA; ADRIANA FARAH GONZALEZ, CNPTIA; VINICIUS MILLEO KUROMOTO, CNPTIA; GUIOMAR ALESSANDRA DE SOUZA TELLES, CNPTIA; RICARDO FONSECA ARAUJO, SIN; CLEIDSON NOGUEIRA DIAS, SIN; BRENO SILVA BEDA DE ASSUNCAO, SIN; SHALON SILVA DE SOUZA FIGUEIREDO, SIN; ARIOVALDO LUCHIARI JUNIOR, CNPTIA; CARLOS ALBERTO ALVES MEIRA, CNPTIA. |
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Título: |
Innovation ecosystem in agriculture: Embrapa's evolution and contributions. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; OLIVEIRA, S. R. de M.; MEIRA, C. A. A.; LUCHIARI JUNIOR, A.; BOLFE, E. L. (ed.). Digital agriculture: research, development and innovation in production chains. Brasília, DF: Embrapa, 2023. cap. 12, p. 209-227. |
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ISBN: |
978-65-89957-72-0 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Debora Pignatai Drucker, Adriana Farah, Ricardo Araújo, Shalon Silva Souza. |
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Conteúdo: |
Introduction. Embrapa's performance record in agricultural innovation ecosystems. Relationships established by Embrapa with ecosystem actors; Relationships of Embrapa Digital Agriculture with the innovation ecosystem; Embrapa's contribution to adopting and evaluating the effect of technologies in agriculture. Developing the agricultural innovation ecosystem in the state of São Paulo. Innovative public-private partnership models for digital innovation in agriculture. Final considerations. |
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Palavras-Chave: |
AgroAPI; AgTechs; API Agritec; API SATVeg; Ecossistema de inovação agrícola brasileiro; Inovação aberta; Inovação agrícola; Inovation; Startups. |
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Thesagro: |
Agricultura; Inovação. |
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Thesaurus NAL: |
Agriculture; Innovation adoption. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156766/1/LV-Digital-agriculture-2023-cap12.pdf
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Marc: |
LEADER 02052naa a2200457 a 4500
001 2156766
005 2023-09-19
008 2023 bl uuuu u00u1 u #d
020 $a978-65-89957-72-0
100 1 $aROMANI, L. A. S.
245 $aInnovation ecosystem in agriculture$bEmbrapa's evolution and contributions.$h[electronic resource]
260 $c2023
500 $aNa publicação: Debora Pignatai Drucker, Adriana Farah, Ricardo Araújo, Shalon Silva Souza.
520 $aIntroduction. Embrapa's performance record in agricultural innovation ecosystems. Relationships established by Embrapa with ecosystem actors; Relationships of Embrapa Digital Agriculture with the innovation ecosystem; Embrapa's contribution to adopting and evaluating the effect of technologies in agriculture. Developing the agricultural innovation ecosystem in the state of São Paulo. Innovative public-private partnership models for digital innovation in agriculture. Final considerations.
650 $aAgriculture
650 $aInnovation adoption
650 $aAgricultura
650 $aInovação
653 $aAgroAPI
653 $aAgTechs
653 $aAPI Agritec
653 $aAPI SATVeg
653 $aEcossistema de inovação agrícola brasileiro
653 $aInovação aberta
653 $aInovação agrícola
653 $aInovation
653 $aStartups
700 1 $aBAMBINI, M. D.
700 1 $aBARIANI, J. M.
700 1 $aDRUCKER, D. P.
700 1 $aMINITTI, A. F.
700 1 $aGONZALEZ, A. F.
700 1 $aKUROMOTO, V. M.
700 1 $aTELLES, G. A. de S.
700 1 $aARAÚJO, R. F.
700 1 $aDIAS, C. N.
700 1 $aASSUNÇÃO, B. S. B. de
700 1 $aSOUZA, S. S. de
700 1 $aLUCHIARI JÚNIOR, A.
700 1 $aMEIRA, C. A. A.
773 $tIn: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; OLIVEIRA, S. R. de M.; MEIRA, C. A. A.; LUCHIARI JUNIOR, A.; BOLFE, E. L. (ed.). Digital agriculture: research, development and innovation in production chains. Brasília, DF: Embrapa, 2023. cap. 12, p. 209-227.
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